| 深海嗜热噬菌体GVE2头尾连接蛋白EV8的原核表达和功能分析 |
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| 引用本文： | 周治东,金春英,吕华,王蔚. 深海嗜热噬菌体GVE2头尾连接蛋白EV8的原核表达和功能分析[J]. 生命科学研究, 2011, 15(2) |
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| 作者姓名： | 周治东 金春英 吕华 王蔚 |
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| 作者单位： | 1. 国家海洋局第三海洋研究所海洋生物遗传资源重点实验室,中国福建厦门,361005
2. 华侨大学化工学院,中国福建厦门,361021 |
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| 基金项目： | 国家自然科学基金资助项目，福建省自然科学基金计划项目，国家海洋局海洋生物遗传资源重点实验室开放研究基金项目，中国科学院海洋生物资源可持续利用重点实验室开放基金项目 |
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| 摘 要： | 为了解头尾连接蛋白在噬菌体感染和装配中起的作用,对高温噬茵体GVE2(virulent bacteriophage of Geobacillus sp.E263)的头尾连接蛋白EV8进行了原核表达和功能鉴定.将EV8编码序列克隆入pGEX4T-2原核表达载体,并转染大肠埃希氏菌BL21.诱导表达后用SDS-PAGE进行鉴定,显示获得相对分子质量约36kD的谷胱甘肽硫转移酶(GST-EV8)融合蛋白.Western blot检测发现EV8在GVE2感染后2h开始表达,说明该蛋白为噬菌体晚期表达蛋白.免疫电镜定位表明头尾连接蛋白位于噬菌体头尾的连接处,为进一步研究高温噬菌体的组装和表达调控奠定了基础.
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| 关 键 词： | 高温噬菌体 头尾连接蛋白 |
Prokaryotic Expression and Functional Characterization of Head-tail Joining Protein EV8 from Thermophilic Bacteriophage GVE2 |
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| ZHOU Zhi-dong,JIN Chun-ying,L Hua,WANG Wei. Prokaryotic Expression and Functional Characterization of Head-tail Joining Protein EV8 from Thermophilic Bacteriophage GVE2[J]. Life Science Research, 2011, 15(2) |
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| Authors: | ZHOU Zhi-dong JIN Chun-ying L Hua WANG Wei |
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| Affiliation: | ZHOU Zhi-dong,JIN Chun-ying,L(U) Hua,WANG Wei |
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| Abstract: | To investigate the role of head-tail joining protein in viral infection and DNA packaging,the head-tail joining protein EV8 from the deep-sea thermophilic bacteriophage GVE2 was characterized.EV8 encoding gene was cloned into prokaryotic expression vector pGEX4T-2 and transformed into E.coli BL21 for expression.The purified proteins characterized by SDS-PAGE and the 36 kD GST-EV8 protein was obtained.The Western blot results demonstrated that the EV8 gene was expressed in the 2 hours of GVE2 infection,imply... |
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| Keywords: | EV8 |
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