Cryoprotective effects of D-allose on mammalian cells |
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Authors: | Sui Li Nomura Rika Dong Youyi Yamaguchi Fuminori Izumori Ken Tokuda Masaaki |
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Institution: | Department of Cell Physiology, Faculty of Medicine, Kagawa University, Kagawa, Japan; Kagawa Industry Support Foundation, Kagawa, Japan. |
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Abstract: | D-allose, an aldo-hexose, is a rare sugar whose biological functions remain largely unclear. Recently, we demonstrated a novel inhibitory effect of D-allose on production of reactive oxygen species (ROS). Here, we focused on investigating cryoprotective effects of D-allose on cell viability. Mammalian cell lines including OVCAR-3 (human ovarian cancer), HeLa (human cervical cancer), HaCaT (human skin keratinocytes), HDF (human dermal fibroblasts) and NIH3T3 (murine fibroblasts) cells were frozen at -80 degrees C in culture media with various D-allose concentrations. Cells were allowed to recover for 24 h, 1 week or 1 month prior to survival assessment using the trypan blue dye exclusion test, when cell proliferation was evaluated by MTT assay. A beneficial protective role of D-allose on cell survival was found, similar to that of trehalose (disaccharide of glucose), a recognized cryoprotectant. The results suggest that D-allose as a sole additive may provide effective protection for mammalian cells during freezing. Practical studies now need to be performed with D-allose, for example to determine optimal freezing protocols and explore potential for preservation of tissues or organs at non-freezing temperatures. |
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Keywords: | Rare sugars d-Allose" target="_blank">d-Allose Cell cryopreservation |
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