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| 细小病毒B19 Oligo探针设计 | |
| 引用本文： | 吕梁,马文丽,孙朝晖,马晓冬,郑文岭.细小病毒B19 Oligo探针设计[J].生物技术通讯,2004,15(1):17-19. |
| 作者姓名： | 吕梁 马文丽 孙朝晖 马晓冬 郑文岭 |
| 作者单位： | 1. 第一军医大学,分子生物学研究所,广州,510515 2. 第一军医大学,分子生物学研究所,广州,510515;广州军区总医院,分子肿瘤学研究所,广州,510010 3. 广州军区总医院,分子肿瘤学研究所,广州,510010 |
| 基金项目： | 广州市重大科技攻关项目(99-Z-022-01) |
| 摘 要： | 利用BLAST软件对细小病毒B19的序列进行序列比对,获得特异序列;利用生物学软件Oligo6.40设计特异性高、Tm值接近、长度均一的Oligo探针。结果获得了13条70bp的Oligo探针,用于芯片打印及细小病毒B19的检测。表明利用BLAST系统和生物学软件Oligo6.40设计细小病毒B19诊断芯片的探针是一种简便而有效的方法。 |
| 关 键 词： | 细小病毒B19 Oligo探针 生物信息学 BLAST |
| 文章编号： | 1009-0002(2004)01-0017-03 |
| 修稿时间： | 2003年7月21日 |
Design of oligonucleotide probes for microarray detection of human parvovirus B19 |
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| LV Liang ,MA Wen-li ,SUN Zhao-hui ,MA Xiao-dong ,ZHENG Wen-ling.Design of oligonucleotide probes for microarray detection of human parvovirus B19[J].Letters in Biotechnology,2004,15(1):17-19. | |
| Authors: | LV Liang MA Wen-li SUN Zhao-hui MA Xiao-dong ZHENG Wen-ling |
| Institution: | LV Liang 1,MA Wen-li 1,SUN Zhao-hui 1,2,MA Xiao-dong 1,ZHENG Wen-ling 2 1.Institute of Molecular Biology,First Military Medical University,Guangzhou510515,2.Institute of Molecular Oncology,Liuhuaqiao Hospital,Guangzhou510010,China |
| Abstract: | To design oligo probes for microarray detection of human parvovirus B19.By analyzing DNA sequence of hu-man parvovirus B19with BLAST program,a group of specific sequences as candidate regions was located.Then Oligo6.40software was applied to select probes with high specificity,identical length and similar melting temperature(Tm).13oligo probes were designed,which will be deposited on chips as a microarray for detection of human parvovirus B19.It is an effective method to design oligo probes of human parvovirus B19using BLAST program and Oligo6.40software. |
| Keywords: | human parvovirus B19 oligonucleotide probe bioinformatics BLAST |
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