GM—CSF基因瘤苗抗肿瘤活性的实验研究

转细胞因子基因的肿瘤细胞疫苗是当今肿瘤免疫－基因治疗研究的热点。本文用逆转录病毒载体将人粒细胞－巨噬细胞集落刺激因子基因转入大鼠Ｗａｌｋｅｒ瘤细胞株ＷＲＣ２５６，经Ｘ－射线灭活制备肿瘤疫苗。经ＰＣＲ和Ｓｏｕｔｈｅｒｎ ｂｌｏｔ检测证实，ＧＭ－ＣＳＦ基因已成功地导入了ＷＲＣ２５６细胞。

EXPERIMENTAL INVESTIGATION ON THE TUMOR VACCINE ENGINEERED WITH hGM-CSF GENE

The gene-transferred tumor vaccine is a new interesting advance in the field of antitumor research. Oberservation was made on the antitumor effects of such vaccine on rat Walker tumor. In our study, it is demonstrated that although the growth of the tumor in untransferred vaccine group (GM-CSF) was similar to that in control group(c), there had a significant difference in the tumor size and weight between gene-transferred vaccine group (GM+) and the control group(c). The pathological examination revealed the massive leukocyte infiltration in the tumor tissue of the gene-transferred vaccine group, but rarely in the control group and untransferred group. Spleen CTL cytotoxicity assay showed that the cytotoxicity of spleen CTL in the gene-transferred vaccine group to WRC256 cells were much more powerful than that in control group. These results suggested that the gene-transferred tumor vaccine have induced an immunoresponse in which specific CTL plays an important role against the tumor in vivo.