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Polyphosphoinositides undergo charge neutralization in the physiological pH range: a 31P-NMR study |
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| Authors: | P A van Paridon B de Kruijff R Ouwerkerk K W Wirtz |
| Abstract: | The charge state of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate was determined as a function of pH by way of 31P-NMR spectroscopy. The pK values for the first protonation of the phosphomonoester residues in phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate were found to be 6.2 and 6.6, respectively, for the 4-phosphate moiety, and 7.7 for the 5-phosphate moiety. |
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