Labelled antibody membrane assay for parathyroid hormone a new approach to the measurement of receptor bound hormone. |
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Authors: | C H McIntosh R D Hesch |
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Institution: | Department of Medicine Endocrinology Section University of Göttingen Germany |
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Abstract: | A new method is described for the measurement of hormone bound to membrane receptors. Antibodies specific for the C-terminal and N-terminal regions of parathyroid hormone were labelled with 125I and incubated with renal membranes which had been previously incubated with unlabelled hormone. The uptake of hormone demonstrated pH and time dependence and was a saturable process. Treatment of the membranes with acid or heating to 100°C, or inactivation of the hormone with hydrogen peroxide, completely abolished detectable hormone uptake to the membranes. |
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