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A large open reading frame ( orf1995 ) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein |
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| Authors: | E. Boudreau M. Turmel M. Goldschmidt-Clermont J.-D. Rochaix S. Sivan A. Michaels S. Leu |
| Affiliation: | (1) Département de Biochimie, Faculté des sciences et de génie, Université Laval, Québec G1K 7P4, Canada, CA;(2) Département de Biologie Moléculaire, Université de Genève, CH-1211, Genève 4, Switzerland, CH;(3) The Doris and Bertie Black Center of Bioenergetics in Life Sciences, Ben Gurion University of the Negev, P.O. Box 653, Beer Sheva, Israel, IL |
| Abstract: | An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival. Received: 18 August 1996 / Accepted: 24 September 1996 |
| Keywords: | Green algae Chlamydomonas reinhardtii Chloroplast genome Gene inactivation Transmembrane protein |
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