Visualization of Plant Tissues by Optical Coherence Tomography |
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Authors: | V. V. Sapozhnikova V. A. Kamenskii R. V. Kuranov |
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Affiliation: | (1) Institute of Applied Physics, Russian Academy of Sciences, ul. Ul'yanova 46, Nizhni Novgorod, 603950, Russia |
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Abstract: | The internal structure of plant tissues was visualized with optical coherence tomography (OCT). This noninvasive method is suitable for examining intact plants; it produces two-dimensional images of plant tissues at a penetration depth of 1–2 mm from the surface. The potential use of OCT was assessed on Tradescantia blossfeldiana Mild. Plant tissue images measuring 1.5 × 2 mm were obtained in vivo with a spatial resolution of 15 m. The radiation power incident on a sample was 0.5 mW. The acquisition of a two-dimensional image consisting of 200 × 200 pixels required 1–3 s. The OCT method can be used to visualize not only plant tissues and tissue boundaries but also the structure of individual cells. |
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Keywords: | Tradescantia blossfeldiana optical coherence tomography tissue structure |
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