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Insolubilized amylases
Authors:Henry M Walton  James E Eastman
Abstract:Carrier-bound immobilized α-amylases and glucoamylase have been obtained by photopolymerizing aqueous solutions containing acrylamide, a crosslinking monomer, an enzyme-reactive monomer, an oxygen scavenger, and amylase, and allowing the amylase to react with the entrapping enzyme-reactive gel matrix evolved. Partial starch hydrolyzates (28 DE corn starch syrup) have been continuously converted to 90–94% dextrose syrups in columns packed with immobilized amylases. The enzymatic heat stability of immobilized glucoamylase gels is discussed.
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