The Determination of d-Glutamic Acid and d-Aspartic Acid by Means of a New Oxidase |
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Authors: | Shôji Mizushima Kazuo Izaki Hajime Takahashi Kin-ichiro Sakaguchi |
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Institution: | Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo |
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Abstract: | The fractional determination of d-glutamic and d-aspartic acids using the enzyme preparation of Aspergillus ustus strain f. was studied. In the first part of this paper, the procedure of enzyme preparation, the effect of sodium chloride on enzyme activity, and a new device for the fractional determination of d-glutamic and d-aspartic acids are described. In the latter part, the contents of d-glutamic and d-aspartic acids of cancer and normal tissues are estimated. However, it was found that the cancer tissues are not characterized by the presence of d-glutamic acid in opposition to Kögl’s claim. |
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