A putative carbohydrate-binding domain of the lactosebindingCytisus sessilifolius anti-H(O) lectin has a similar amino acid sequence to that of thel-fucose-bindingUlex europaeus anti-H(O) lectin |
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| Authors: | Yukiko Konami Kazuo Yamamoto Toshiaki Osawa and Tatsuro Irimura |
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| Institution: | (1) Department of Cancer Biology and Molecular Immunology (formerly Division of Chemical Toxicology and Immunochemistry), Faculty of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, 113 Tokyo, Japan;(2) Present address: Yakult Central Institute for Microbiological Research, Yaho, Kunitachi-shi, Tokyo |
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| Abstract: | The complete amino acid sequence of a lactose-bindingCytisus sessilifolius anti-H(O) lectin II (CSA-II) was determined using a protein sequencer. After digestion of CSA-II with endoproteinase Lys-C or Asp-N, the resulting peptides were purified by reversed-phase high performance liquid chromatography (HPLC) and then subjected to sequence analysis. Comparison of the complete amino acid sequence of CSA-II with the sequences of other leguminous seed lectins revealed regions of extensive homology. The amino acid sequence of a putative carbohydrate-binding domain of CSA-II was found to be similar to those of several anti-H(O) leguminous lectins, especially to that of thel-fucose-bindingUlex europaeus lectin I (UEA-I).Abbreviations BPA
Bauhinia purpurea lectin
- Con A
concanavalin A
- CMA-I
Cytisus multiflorus lectin I
- CMA-II
Cytisus multiflorus lectin II
- CSA-I
Cytisus sessilifolius lectin I
- CSA-II
Cytisus sessilifolius lectin II
- CSII
Cytisus scoparius lectin II
- ECorL
Erythrina corallodendron lectin
- GSIV
Griffonia simplicifolia lectin IV
- HPLC
high performance liquid chromatography
- LAA-I
Laburnum alpinum lectin I
- LAA-II
Laburnum alpinum lectin II
- LOL
Lathyrus ochrus lectin
- LTA
Lotus tetragonolobus lectin
- MAH
Maackia amurensis haemagglutinin
- PSA
Pisum sativum lectin
- SDS
sodium dodecyl sulfate
- TFA
trifluoroacetic acid
- UEA-I
Ulex europaeus lectin I
- UEA-II
Ulex europaeus lectin II
- VFA
Vicia faba lectin |
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| Keywords: | lactose-binding anti-H(O) lectin lectin primary structure |
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