UV-B预处理诱导拟南芥耐旱性的提高

为了解UV-B提高拟南芥(Arabidopsis thaliana)耐旱性的生理机制,将2周龄的野生型拟南芥(WT)和sto突变体幼苗用不同剂量UV-B预处理1周,再用30%PEG模拟干旱处理24 h,对植株的表型进行统计,并测定类黄酮、脯氨酸和MDA含量。结果表明,低剂量UV-B预处理能够提高拟南芥的耐旱性,植株的类黄酮与脯氨酸含量分别提高了20%～40%和50%～65%,细胞膜受损程度降低,从而提高了保水性。低剂量UV-B提高拟南芥耐旱性的效应在sto突变体中消失,证明这种效应在分子机制上可能与STO蛋白相关。

UV-B-induced Drought Tolerance Improvement in Arabidopsis thaliana

In order to understand the physiological mechanism of UV-B to improve drought resistance of Arabidopsis thaliana, the wild type (Columbia-0, WT) and sto mutant seedlings at 2-week-old were treated with UV-B for one week, and then the seedlings were treated with 30%PEG for 24 hours, the phenotypes of the plants were counted, and the contents of flavonoids, proline and MDA were determined. The results showed that low doses of UV-B pretreatment could enhance drought resistance of A. thaliana, the contents of proline and flavonoids increased for 20%-40% and 50%-65%, respectively, and the cell membrane damage reduced, the water retention was improved. The effect of low dose UV-B in enhancing A. thaliana drought tolerance disappeared in sto mutants, indicating the effect might be related to STO protein in the molecular mechanism.