MiR-124对先天性甲低新生鼠神经元凋亡的影响

目的:探讨mi R-124在甲状腺功能低下新生大鼠海马神经元凋亡中的作用。方法:将16只孕SD雌鼠随机分为实验组和对照组,分别于两组仔鼠1、5、10、15日龄称重取血,用化学发光法测定血清游离三碘甲状腺原氨酸(FT3)、血清游离甲状腺素(FT4)及促甲状腺激素(TSH)水平。将大鼠用2%戊巴比妥钠腹腔注射麻醉后,固定在脑立体定位仪,处死后收集其海马,应用荧光定量PCR和免疫荧光技术检测agomir的干预效率,确定干预有效后进行建模。观察注射后大鼠行为学改变,NeuN染色法检测海马区脑组织神经元细胞凋亡情况。结果:实验组血清TSH水平明显高于对照组,而FT3、FT4水平明显低于对照组。同正常对照组相比,甲状腺功能减退组中阳性神经元数量明显减少、细胞轮廓模糊、不清晰。经mir-124模拟处理后,神经细胞的数量和形态明显改善。结论:mi R-124对先天性甲状腺功能低下新生鼠神经元凋亡起到保护作用。

The Effect of MIR-124 on the Neurons Apoptosis of Neurons in the Newborn Rats with Congenital Hypothyroidism

ABSTRACT Objective: To investigate the effect of miR-124 on the neuronal apoptosis induced by congenital hypothyroidism. Methods: 16 female SD rats were randomly divided into the experimental group and the control group, the blood samples were collected from both groups of rats at 1, 5, 10 and 15 days, respectively. The serum levels of FT3, FT4 and TSH were measured by chemiluminescence method. The rats were anesthetized with injection of 2% pentobarbital sodium, fixed in brain stereo positioning instrument,the hippocampus was collected from the mouse after being killed. The efficiency of agomir intervention was detected by fluorescence quantitative PCR and immunofluorescence technique, and the model was established after the intervention was effective. The behavioral changes of rats were observed, and apoptosis of brain tissue neurons in hippocampus was detected by NeuN staining. Results: The level of serum TSH in the experimental group was significantly higher than that in the control group, while the levels of FT3 and FT4 in the experimental group were significantly lower than those in the control group. Compared with the normal control group, the number of positive neurons in the hypothyroid group was significantly decreased, and the outline of the cells was obscure and unclear. After miR-124 treatment, the number and morphology of nerve cells were significantly improved. Conclusion: Mir-124 protects neuronal apoptosis in neonatal rats with congenital hypothyroidism.