生物钟基因PER2在结直肠癌中的表达及意义

目的:探讨PER2基因表达水平和结直肠癌发生发展的关系。方法:收集203例在上海市第一人民医院接受根治性肠切除术结直肠癌患者的标本,通过实时定量PCR和免疫组织化学技术检测PER2在肿瘤组织和邻近正常组织中的表达水平,并对PER2的表达与患者的病理资料和临床预后的相关性进行统计学分析。结果:PER2在结直肠患者肿瘤组织的表达较正常组织减少(P0.001)。与PER2阳性患者相比,PER2阴性的结直肠癌患者有远处转移(P=0.026)、AJCC分期为IV期(P=0.011)的比例更高。结论:PER2基因在结直肠癌患者中存在低表达现象,其对于患者的AJCC分期评价以及了解患者有无远处转移有一定的参考价值。

Expression and Significance of Clock Gene PER2 in Colorectal Cancer

ABSTRACT Objective: To investigate the expression of PER2 and its correlation with the development of colorectal cancer. Methods: A total of 203 cases of colorectal cancer patients who underwent radical enterectomy in Shanghai General Hospital were included. By real- time PCR and immunohistochemistry, PER2 expression in the tumor tissue and adjacent normal tissue were detected, and the correlation of PER2 expression with the pathological characterstics and clinical outcome were statistically analyzed. Results: PER2 expression in tumor was significantly lower than that of the normal tissue in colorectal cancer patients(P<0.001). As compared to PER2-positive tumors, a larger proportion of colorectal cancer patients with PER2-negative tumors had distant metastases(P=0.026) and were AJCC stage IV(P=0.011). Conclusion: PER2 gene expression decrease in colorectal cancer patients, and PER2 may be helpful for AJCC staging of the patients and assessing whether they have tumor metastasis or not.