前列腺癌细胞系中环状RNA circRNA-1565的表达及鉴定

目的:探讨前列腺癌细胞系中的环状RNA circRNA-1565的表达及鉴定。方法:培养前列腺正常上皮细胞(RWPE-1)、4种前列腺癌细胞(22RV1、LNCap、PC-3、DU145),提取总RNA,采用RT-PCR检测不同细胞系中circRNA-1565的表达,并对其进行成环性验证及细胞内亚定位实验,进行差异比较。结果:circRNA-1565在正常前列腺细胞系(RWPE-1)中表达量极低,在转移性前列腺癌细胞系中高表达,在非转移性前列腺癌细胞系中低表达。且circRNA-1565是一条主要定位于细胞质内的具有有效环状结构的RNA分子。结论:circRNA-1565在不同前列腺癌细胞系中存在差异表达,可能会通过mi RNA sponge途径发挥生物学作用。

Expression and Identification of Ring RNA CircRNA-1565 in Prostate Cancer Cell Line

ABSTRACT Objective: To investigate the expression and identification of annular RNA circRNA-1565 in the cell line of prostate cancer. Methods: Normal prostate epithelial cells (RWPE-1), 4 kinds of prostate cancer cells (22RV1, LNCap, PC-3, DU145) were cultured, total RNA was extracted, and RT-PCR was used to detect the expression of circRNA-1565 in different cell lines. The cyclic verification and intracellular localization experiments were carried out to compare the differences. Results: The expression of circRNA-1565 in normal prostate cell line (RWPE-1) was very low, expressed highly in metastatic prostate cancer cell line, and low in non-metastatic prostate cancer cell line. And circRNA-1565 is a RNA molecule with an effective annular structure, which is mainly located in the cytoplasm. Conclusion: The expression of circRNA-1565 in different prostate cancer cell lines may play a biological role through the sponge pathway of miRNA.