罗伊氏乳杆菌对组胺引起黄颡鱼肝肠损伤修复效果评价 |
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引用本文: | 成艳波,李薇,韩木兰,许国焕,谢黎炜,印遇龙,梁建庆. 罗伊氏乳杆菌对组胺引起黄颡鱼肝肠损伤修复效果评价[J]. 水生生物学报, 2019, 43(1): 94-101. DOI: 10.7541/2019.012 |
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作者姓名: | 成艳波 李薇 韩木兰 许国焕 谢黎炜 印遇龙 梁建庆 |
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作者单位: | 广东省微生物研究所广东省部共建华南应用微生物国家重点实验室广东省微生物应用新技术公共实验室;广东碧德生物科技有限公司;中国科学院亚热带农业生态研究所 |
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摘 要: | 以黄颡鱼(Pelteobagrus fulvidraco)(14.23±0.65) g为实验对象, 分别饲喂3组等氮饲料(C组: 组胺含量147 mg/kg; H组: 479 mg/kg和H+B组: 496 mg/kg+1×105 CFU/g罗伊氏乳杆菌Lactobacillus reuteri), 进行56d的养殖实验, 研究组胺及罗伊氏乳杆菌对黄颡鱼的影响。结果显示, H+B组血清谷丙转氨酶(ALT)及谷草转氨酶(AST)活力显著低于H组(P<0.05), 而与C组无差异(P>0.05); H+B组肝脏谷胱甘肽S转移酶(GSH-ST)活力及丙二醛(MDA)含量显著低于H组(P<0.05), 与C组无差异(P>0.05); 肝脏HE染色显示, H组肝脏中有较多炎症细胞浸润, 而H+B组肝脏细胞结构完整, 无明显损伤; 肠道HE染色显示, H组肠道皱襞数量明显减少、长度显著变短, 有水肿发生, 而H+B组肠道皱襞数量、长度与对照组无差异, 水肿程度不明显。结果表明: 组胺会引起黄颡鱼肝脏、肠道损伤, 而罗伊氏乳杆菌对组胺引发的肝肠损伤有保护功效。
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关 键 词: | 黄颡鱼 罗伊氏乳杆菌 组胺 HE染色 保护功效 |
收稿时间: | 2017-12-15 |
THE ENTEROHEPATIC PROTECTION OF PELTEOBAGRUS FULVIDRACO ADDING LACTOBACILLUS REUTERI INDUCED BY HISTAMINE |
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Abstract: | To investigate the effects of histamine and Lactobacillus reuteri on Pelteobagrus fulvidraco, three isonitrogenous diets, namely diet C (control, with a 147 mg/kg content of histamine), diet H (with a 479 mg/kg content of histamine) and diet H+B (histamine+ bacteria: a 496 mg/kg content of histamine and 1×105 CFU/g L. reuteri), were formulated for a 56-day trial of P. fulvidraco juveniles (14.23±0.65) g. Our data indicated that plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were significantly lower in group H+B compared with those of group H (P<0.05), while there was no significant difference between group H+B and group C (P>0.05). Meanwhile, liver glutathione-s-transferase (GSH-ST) activity and malondialdehyde (MDA) content were dramatically reduced in group H+B compared with those of group H (P<0.05), while there was no significant difference between group H+B and group C (P>0.05). Furthermore, the HE staining showed that there were inflammatory cells infiltrating in the liver of fish fed with diet H, while no hepatocyte abnormality observed in group C and group H+B, and that the intestinal folds decreased significantly with shorter and edema in diet H group, while this is no marked difference in the length of intestinal folds between group C and H+B. Overall, our studies indicated the protective effect ofL. reuteri against histamine in cultured fish diet. |
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