辣椒叶多糖抗氧化作用研究

目的:从辣椒叶中分离纯化活性多糖并考察其抗氧化活性。方法:采用水提醇沉法提取多糖,Sevag法脱蛋白,得辣椒叶粗多糖;分别使用超纯水、0.06 mol/L NaCl溶液、0.18 mol/L NaCl溶液作为洗脱液,通过DEAE-52离子交换柱色谱纯化得到三种辣椒叶多糖LD-0、LD-0.06、LD-0.18,测定多糖含量。DPPH、ABTS法检测多糖体外抗氧化作用。以小鼠血清、肝组织中总超氧化物歧化酶活性、过氧化氢酶活性及丙二醛含量为指标,考察小鼠灌胃LD-0.06多糖对脂质过氧化模型的影响。结果:辣椒叶粗多糖、LD-0、LD-0.06、LD-0.18多糖含量分别为9.92%、43.14%、82.97%、37.63%,其中LD-0.06多糖含量最高。体外抗氧化实验结果显示,三种结果均具备较好的清除能力,其中LD-0.06对ABTS+、DPPH·的清除效果最好,IC50值分别为0.58 mg/m L和0.60 mg/m L,结果与对照组在0.05水平具有显著性差异,说明辣椒叶多糖提取物是一种有效的天然抗氧化剂。在脂质过氧化模型小鼠体内,与模型组比较,LD-0.06多糖能显著增强小鼠血清和肝组织中的T-SOD与CAT活性,降低MDA的含量,且剂量越高,体内抗氧化能力越强。结论:辣椒叶多糖提取物具有一定的抗氧化作用,为进一步开发利用辣椒资源提供了理论依据。

Study on Antioxidation of Capsicum annuum Leaves Polysaccharide

ABSTRACT Objective: To extract, purify polysaccharids from Capsicum leaves and to determinate the antioxidant activity. Methods: Crude Capsicum leaves polysaccharide was gained by boiling in hot-water, precipitating using ethanol, removing proteins with Sevage method. Three kinds of Capsicum leaves polysaccharides LD-0, LD-0.06, LD-0.18 were purified through DEAE-52 cellulose colume, us- ing ultrapure water, 0.06 mol/L NaCl solution and 0.18 mol/L NaCl solution as eluent respectively. The content of polysaccharides was determined by the experiments of ABTS radical and DPPH radical scavenging. The effects of low, medium and high dose LD-0.06 polysaccharides on lipid peroxidation model in mice were studied, the indicators included total superoxide dismutase, catalase and mal- ondialdehyde. Results: The contents of polysaccharides, LD-0, LD-0.06 and LD-0.18 were 9.92%, 43.14%, 82.97% and 37.63% respec- tively. Among them, LD-0.06 has the highest polysaccharide content. Antioxidant activity tests revealed that all three methods have good scavenging ability, and LD-0.06 has the best effect on ABTS and DPPH. The IC₅₀ values were 0.58 mg/mL and 0.60 mg/mL, respectively. Compared with the model group, LD-0.06 polysaccharide can significantly enhance the activity of T-SOD and CAT in serum and liver tissue of mice, reduce the content of MDA, and has a significant dose-effect relationship. Conclusion: Capsicum leaf polysaccharide ex- tract has certain antioxidant effect. This study indicated that polysaccharide extract of Casicum annuum leaves had high anti-oxidant ac- tivity, which provided a theoretical basis for the further research of the comprehensive development of Caspsicum annuum resources.