外源性的电磁干预对神经病理性疼痛大鼠的镇痛效果研究

目的:探讨外源性的电磁干预方法对神经病理性疼痛大鼠的镇痛效果。方法:将30只成熟的雄性SD大鼠随机等分成3组:空白对照组(Control),坐骨神经慢性压迫损伤(CCI)组以及坐骨神经慢性压迫损伤协同电磁刺激组(CCI+EMF)。CCI组和CCI+EMF组的20只大鼠建立坐骨神经慢性压迫损伤模型,CCI+EMF组大鼠行外源性的全身性电磁刺激干预(脉冲波形,频率15 Hz,强度30 Gs),每天刺激6小时。在CCI模型构建的第0、3、6、9、12及15天对大鼠测试和比较足底机械痛阈值、足底热痛阈值、运动功能评分和神经传导速率。结果:CCI组大鼠的足底机械痛阈值、足底热痛阈值及感觉神经传导速率从CCI手术后的第3天即出现显著性降低,其6、9、12、15天足底机械痛阈值、足底热痛阈值及感觉神经传导速率均显著低于Control组(P0.01),而运动功能评分均显著高于Control组(P0.05)。CCI+EMF组大鼠的足底机械痛阈值、足底热痛阈值及感觉神经传导速率在第9、12、15天显著高于CCI组大鼠(P0.05),而运动功能评分均显著高于CCI l组。结论:外源性的电磁刺激对于神经病理性疼痛大鼠具有良好的镇痛效果,有望成为一种临床治疗神经病理性疼痛的新的物理治疗手段。

Investigation for the Effect of Exogenous Electromagnetic Stimulation on the Analgesia in Rats with Neuropathic Pain

ABSTRACT Objective: To identify the effects of exogenous electromagnetic stimulation on the analgesia in rats with neuropathic pain. Methods: Thirty male adult SD rats were randomly and equally assigned into the blank control (Control), chronic constriction injury (CCI) and chronic constriction injury with electromagnetic stimulation (CCI+EMF) groups. The rats in CCI group and CCI+EMF group were subjected to the surgery for CCI, and the rats in CCI+EMF group were subjected to whole-body electromagnetic stimulation with 6 h/day (sinusoidal waveform, 15 Hz frequency and 30 Gs intensity). The paw mechanical pain threshold, paw thermal pain threshold, motor function score and nerve conduction velocity were determined and compared on the day 0, 3, 6, 9, 12 and at 15 days post the CCI surgery. Results: The paw mechanical pain threshold, thermal pain threshold and sensory nerve conduction velocity of CCI group were significantly decreased from day 3. The paw mechanical pain threshold, thermal pain threshold and sensory nerve conduction velocity in the CCI group were significantly lower than those in those of the control group (P<0.01), and the motor function of CCI rats was significantly higher than that in the control group (P<0.05). Moreover, the paw mechanical pain threshold, thermal pain threshold and sensory nerve conduction velocity of CCI+EMF group on day 9, 12 and 15 were significantly higher than those in the CCI group (P<0.05), and the motor function of CCI+EMF group was significantly lower than that of the control group (P<0.05). Conclusion: Exogenous electromagnetic stimulation exhibited excellent analgesia effects in rats with neuropathic pain, and highlighted that electromagnetic stimulation may become a novel biophysical strategy for the treatment of neuropathic pain in clinic.