早孕因子单克隆抗体对黑色素瘤细胞A-375增殖、凋亡的影响

目的:研究早孕因子单克隆抗体(EPF-McAb)对黑色素瘤细胞A-375增殖、凋亡的影响。方法:体外培养黑色素瘤细胞A-375,通过CCK-8实验检测EPF-McAb对A-375细胞增殖的影响;通过流式细胞术检测EPF-McAb对A-375细胞凋亡的影响;Western Blot检测EPF-McAb对A-375细胞EPF蛋白表达的影响。结果:CCK-8结果显示EPF-McAb可以抑制黑色素瘤细胞A-375的增殖,且随着作用时间延长和药物浓度的增加,对A-375细胞增殖的抑制作用也增强;流式细胞术实验结果显示EPF-McAb可以促进黑色素瘤细胞A-375的凋亡,且调亡率随着药物浓度的增加而升高,0.2、0.4、0.8 mg/m L的EPF单抗作用于A-375细胞24 h后,细胞调亡率分别为:14.68%(P0.01)、19.81%(P0.01)、23.97%(P0.01);Western Blot实验结果显示EPF-McAb可以降低黑色素瘤细胞A-375 EPF蛋白的表达。结论:早孕因子单克隆抗体可以抑制黑色素瘤细胞A-375的增殖,并促进其凋亡。

Effect of Monoclonal Antibody against Early Pregnancy Factor on Proliferation and Apoptosis of Melanoma cell A-375

ABSTRACT Objective: To study the effect of early pregnancy factor monoclonal antibody (EPF-McAb) on proliferation and apop- tosis of melanoma cell A-375. Methods: CCK-8 experiment, flow cytometry and Western Blot were conducted on cultured melanoma cells A-375 in vitro to understand the effect of EPF-McAb on proliferation, apoptosis and protein expression of A-375 cells respectively. Results: CCK-8 results showed that EPF-McAb could inhibit the proliferation of A-375 in melanoma cells, and the effect was also en- hanced with the prolongation of action time and the increase of drug concentration. The results of flow cytometry showed that EPF-McAb could promote the apoptosis of A-375 in melanoma cells, and the rate of apoptosis was associated with the drug concentra- tion. The increase of 0.2, 0.4, 0.8 mg/mL EPF monoclonal antibody on A-375 cells 24 h, the cell apoptosis rate was 14.68% (P<0.01), 19.81% (P<0.01), 23.97% (P<0.01); Western Blot experimental results showed that EPF-McAb can reduce the expression of A-375 EPF protein in melanoma cells. Conclusion: EPF-McAb can not only inhibit the proliferation of melanoma cell A-375, but also promote its apoptosis.