| 黄芪红花配伍对大鼠脑缺血后血管新生及Cav-1/VEGF信号通路的影响 |
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| 引用本文： | 曹金一,雷 露,段佳林,胡咏武,乔 逸,文爱东,杨志福.黄芪红花配伍对大鼠脑缺血后血管新生及Cav-1/VEGF信号通路的影响[J].现代生物医学进展,2019,19(6):1016-1019. |
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| 作者姓名： | 曹金一 雷 露 段佳林 胡咏武 乔 逸 文爱东 杨志福 |
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| 作者单位： | 空军军医大学西京医院药剂科;中国药科大学基础医学与临床药学学院 |
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| 基金项目： | 国家自然科学基金项目（81503280，81573549） |
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| 摘 要： | 目的:黄芪红花配伍是否通过调节小凹蛋白1(Caveolin-1,Cav-1)/血管内皮生长因子(vascular endothelial growth factor,VEGF)通路促进血管新生保护大鼠脑缺血损伤。方法:60只雄性SD大鼠随机分为5个组:对照组(Sham组,n=12),模型组(MACO组,n=12),黄芪红花40:1组(n=12),20:1组(n=12),5:1(n=12)。大鼠脑缺血再灌注损伤模型采用尼龙线栓法制作,连续给药21d后,评价神经功能学评分,计算脑梗死体积,采用免疫组化法测定皮质区的微血管密度,采用RT-PCR法检测皮质区VEGF m RNA和Cav-1 m RNA表达,采用Western-blotting法测定皮质区VEGF和Cav-1的蛋白表达。结果:连续给药21d后,各组大鼠的神经功能学评分均有所降低,3个不同比例的黄芪红花组的神经功能学评分降低最为明显(P0.01),脑梗死体积较模型组显著减少(P0.05~P0.01),微血管密度、VEGF和Cav-1 m RNA和蛋白表达水平均较模型组明显升高(P0.05~P0.01)。结论:黄芪红花配伍可能通过调节Cav-1/VEGF信号通路促进脑缺血再灌注损伤大鼠脑内的血管新生,从而减轻脑缺血损伤,且最佳的配伍比例为黄芪红花5:1。
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| 关 键 词： | 黄芪 红花 脑缺血再灌注 血管新生 小凹蛋白1 血管内皮生长因子 |
| 收稿时间： | 2018/9/20 0:00:00 |
| 修稿时间： | 2018/10/13 0:00:00 |
Effect of Huangqi-honghua Combination on the Angiogenesis and Cav-1/VEGF Signal Pathway in Brain Tissue of Rats with Cerebral Ischemia Reperfusion Injury |
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| CAO Jin-yi,LEI Lu,DUAN Jia-lin,HU Yong-wu,QIAO Yi,WEN Ai-dong and YANG Zhi-fu.Effect of Huangqi-honghua Combination on the Angiogenesis and Cav-1/VEGF Signal Pathway in Brain Tissue of Rats with Cerebral Ischemia Reperfusion Injury[J].Progress in Modern Biomedicine,2019,19(6):1016-1019. |
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| Authors: | CAO Jin-yi LEI Lu DUAN Jia-lin HU Yong-wu QIAO Yi WEN Ai-dong and YANG Zhi-fu |
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| Institution: | Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi''an, Shaanxi, 710032, China,School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China,Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi''an, Shaanxi, 710032, China,Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi''an, Shaanxi, 710032, China,Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi''an, Shaanxi, 710032, China,Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi''an, Shaanxi, 710032, China and Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi''an, Shaanxi, 710032, China |
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| Abstract: | |
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| Keywords: | Huangqi Honghua Ischemia reperfusion injury Angiogenesis Caveolin-1 Vascular endothelial growth factor |
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