肿瘤相关成纤维细胞对非小细胞肺癌恶性生物学行为影响

目的:探讨肿瘤相关成纤维细胞(Tancer Associated Fibroblast,TAF)对非小细胞肺癌(Non-small Cell Lung Cancer,NSCLC)恶性生物学行为的影响。方法:选取在本院肿瘤科住院手术的非小细胞肺癌患者,收集术后肺癌标本,马松三色染色(Masson Trichrome Stain)和天狼星红染色(Sirius Red Stain)观察肺癌组织(Lung Cancer Tissue,LCT)、癌旁组织(Pericarcinomatous Tissue,PCT)和正常组织(Normal Tissue,NT)中TAF的表达情况;体外将非小细胞肺癌细胞A549与非小细胞肺癌成纤维细胞P-gp共培养,CCK-8检测共培养前后A549细胞增殖能力;细胞划痕和Trans-well实验分别检测A549细胞迁移和侵袭能力;q RT-PCR和Western blot检测A549细胞上皮间质转化(Epithelial Mesenchymal Transition,EMT)标志蛋白E-cadherin、N-cadherin和Vimentin的表达。结果:Masson和Sirius染色结果显示:肺癌组织中纤维的表达明显高于癌旁组织;与P-gp共培养的A549细胞的增殖、迁移和侵袭能力及上皮间质转化相关蛋白N-cadherin和Vimentin表达均明显高于阴性对照组(P0.05),而E-cadherin的表达明显降低(P0.05)。结论:TAF可能通过诱导非小细胞肺癌细胞EMT的发生从而促进非小细胞肺癌的增殖、迁移和侵袭等恶性生物学行为。

Effect of Tumor Associated Fibroblasts on the Malignant Biological Behavior of Non-small Cell Lung Cancer

ABSTRACT Objective: To investigate the effect of tumor-associated fibroblast (TAF) on the malignant biological behavior of non-small cell lung cancer (NSCLC). Methods: Patients with non-small cell lung cancer who were hospitalized in our oncology department were enrolled. Postoperative lung cancer specimens, Masson trichrome stain and sirius red stain were used to observe the lung cancer tissue (Lung Cancer Tissue, Expression of TAF in LCT), Pericarcinomatous tissue (PCT) and normal tissue (NT); the non-small cell lung cancer cell A549 was co-cultured with non-small cell lung cancer fibroblast P-gp in vitro, CCK8 was used to detect the proliferation of A549 cells; cell scratch and Trans-well assays were used to detect the migration and invasion of A549 cells; qRT-PCR and Western blot were used to detect the expression of epithelial mesenchymal transition (EMT) markers such as E-cadherin, N-cadherin and Vimentin in A549 cells. Results: Masson and Sirius staining showed that the expression of fiber in lung cancer tissues was significantly higher than that in the adjacent tissues. The proliferation, migration and invasion ability of A549 cells co-cultured with P-gp and the expression of N-cadherin and Vimentin were significantly higher than those of the negative control group (P<0.05), while the expression of E-cadherin was significantly decreased (P<0.05). Conclusion: TAF may promote the malignant biological behaviors such as proliferation, migration and invasion of non-small cell lung cancer by inducing the occurrence of EMT in the non-small cell lung cancer cells.