| 环磷酰胺对大鼠神经上皮细胞向神经元方向分化的影响 |
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| 引用本文： | 陈燕春,管英俊,刘焕彩,于丽,赵春艳.环磷酰胺对大鼠神经上皮细胞向神经元方向分化的影响[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 陈燕春 管英俊 刘焕彩 于丽 赵春艳 |
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| 作者单位： | 1. 潍坊医学院组胚教研室,山东,261042
2. 潍坊医学院附属医院 |
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| 基金项目： | 山东省科技攻关计划项目 |
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| 摘 要： | 目的研究环磷酰胺对大鼠神经上皮细胞向神经元方向分化的影响,进一步探讨其致畸作用的分子机制,为预防人类NTDs的发生提供理论依据。方法将孕鼠随机分实验组和对照组,实验组在妊娠第13天上午8-9时腹腔注射环磷酰胺(15mg/kg体重),对照组注射等量生理盐水。分别于给药后4、8、12、24、48h处死孕鼠,剖腹取出胎鼠,于10%中性福尔马林中固定,常规石蜡包埋、连续切片。应用免疫组织化学和免疫荧光技术检测β-tubulin和NeuN在NTDs发生中的表达变化。结果(1)β-tubulin免疫荧光染色结果:给药后4、8h,实验组β-tubulin阳性率与对照组相比,差异无统计学意义;给药后12、24、48h,β-tubulin阳性细胞与对照组相比减少,差异均有统计学意义。(2)NeuN免疫组织化学结果:给药后4、8、12h,实验组和对照组均未检测到NeuN阳性细胞;给药后24、48h,实验组与对照组相比,NeuN阳性细胞减少,差异均有统计学意义。结论环磷酰胺可使β-tubulin、NeuN的表达降低,干扰神经上皮细胞向神经元方向分化。
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| 关 键 词： | 环磷酰胺 神经管畸形 β-Tubulin NeuN |
INFLUENCE OF CYCLOPHOSPHAMIDE ON THE DIFFERENTIATION OF RAT NEUROEPITHELIAL CELLS INTO NEURONS |
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| Chen Yanchun,Guan Yingjun,Liu Huancai,Yu Li,Zhao Chunyan.INFLUENCE OF CYCLOPHOSPHAMIDE ON THE DIFFERENTIATION OF RAT NEUROEPITHELIAL CELLS INTO NEURONS[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Chen Yanchun Guan Yingjun Liu Huancai Yu Li Zhao Chunyan |
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| Institution: | 1Department of Histology and Embryology;Weifang Medical College;2Affiliated Hospital of Weifang Medical College;Shandong 261042;China |
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| Abstract: | Objective To define the influence of cyclophosphamide on the differentiation of rat neuroepithelial cells into neurons, and to further explore the mechanism that causes neural tube defects in order to provide a theoretical basis for the prevention of human NTDs.Methods Pregnant rats were randomly divided into the experimental group and the control group. At 8 am to 9 am on the 13th day of pregnancy, the experimental group was intraperitoneally injected with cyclophosphamide (15mg/kg body weight), and the co... |
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| Keywords: | NeuN |
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