Transglycosylation reactions by exoglycosidases from the termite Macrotermes subhyalinus |
| |
Authors: | Lucien Patrice Kouamé Sébastien Niamké Jacques Diopoh Bernard Colas |
| |
Affiliation: | (1) Laboratoire de Biochimie, Unité de Recherche sur la Biocatalyse, CNRS 2230, Faculté des Sciences et des Techniques, 2 rue de la Houssinière, BP 92208, F 44322 Nantes cedex 3, France;(2) Département de Biochimie, Université d'Abidjan, 22 BP 522, Abidjan, Côte d'Ivoire |
| |
Abstract: | The ability of four exoglycosidases (-galactosidase, -glucosidase, -glucosidase and invertase) from the termite Macrotermes subhyalinus to catalyse tranglycosylation reactions was tested using lactose, cellobiose, maltose and sucrose as glycosyl donors and 2-phenylethanol as glycosyl acceptor. The experimental conditions were optimized in relation to the time course of the reaction, pH and concentrations of glycosyl donor and acceptor. Whereas the hydrolytic activity was largely predominant over the transferase activity with -galactosidase and -glucosidase, the transglycosylation activity represented 68% with -glucosidase. In addition, as demonstrated by the transglycosylation product formed, the hydrolysis of sucrose was catalysed by -glucosidase and not by invertase. On the basis of this work, -glucosidase from M. subhyalinus appears to be a valuable tool for the preparation of neoglycoconjugates. |
| |
Keywords: | /content/k492l281t458v720/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-galactosidase /content/k492l281t458v720/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >-glucosidase /content/k492l281t458v720/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-glucosidase invertase Macrotermes subhyalinus termites transglycosylation |
本文献已被 SpringerLink 等数据库收录! |
|