Biological and immunological characterization of purified follicle-stimulating hormone preparations from monkey pituitaries |
| |
Authors: | Shafiq A Khan Berit Frysa Egon Diczfalusy |
| |
Institution: | Shafiq A. Khan,Berit Fröysa,Egon Diczfalusy |
| |
Abstract: | The suitability of a purified rhesus (rhFSH) and cynomolgus (cynFSH) follicle-stimulating hormone (FSH) preparation to replace the expired LER 1909-2 reference standard in the immunoassay of monkey FSH was assessed using an in vitro bioassay and two heterologous radioimmunoassay (RIA) procedures in combination with fractionation by high-resolution isoelectrofocusing. The results presented indicate that rhFSH is unsuitable for the replacement of LER 1909-2 as a standard, though cynFSH might be suitable after the removal of its LH contamination. |
| |
Keywords: | Monkey FSH Radioimmunoassay standards Purification Bioactivity Isoelectrofocusing Microheterogeneity |
本文献已被 PubMed 等数据库收录! |