Protoplast culture and somaclonal variability of species of series Juglandifolia |
| |
Authors: | A S Kochevenko Y I Ratushnyak Y Y Gleba |
| |
Institution: | (1) Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Zabolotnogo str. 148, 252022, DSP-22 Kiey, Ukraine;(2) Present address: American Cyanamid Company, P.O.B. 400, 08543-0400 Princeton, NJ, USA |
| |
Abstract: | Mesophyll protoplasts of species of series Juglandifolia (Solanum rickii, S. lycopersicoides, S. ochranthum and S. juglandifolium) were isolated and cultured in liquid nutrient media TM-2 or KM8P. The cell colonies formed were transferred onto agar-solidified media TM-3 or GM, and 10 to 15 days later onto TM-4, PRM, MS3ZG, KK or C regeneration media. Formation of the shoots for S. rickii and S. lycopersicoides was observed after 30 to 35 days on regeneration medium. The regenerated shoots were rooted on hormone-free MS medium. Morphological and cytogenetic analyses have shown that somaclonal variants might arise in the course of plant regeneration from protoplasts of these species.Abbreviations BAP
6-benzylaminopurine
- 2, 4-D
2, 4 dichlorophenoxyacetic acid
- NAA
-naphthaleneacetic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog medium
- B5
Gamborg medium
- TRIS
tris(hydroxymethyl)aminomethane |
| |
Keywords: | Chromosome numbers leaf morphology organogenesis protoplast culture series Juglandifolia somaclonal variability |
本文献已被 SpringerLink 等数据库收录! |
|