Nestmate recognition in the leaf-cutting antAtta laevigata

Summary We tested matureAtta laevigata colonies in the field to see if the ants used queen substances, environmental odours (in this case odours produced by the nest's fungi), an odour produced by each individual, or a gestalt odour (resulting from odours distributed between nestmates) as a discrimination signal for nestmate recognition. We found that nestmate recognition inA. laevigata appears to be largely based on an odour produced by each nestmate which appears to be concentrated in the head, although other odours may also be used. We found no evidence of genetic relatedness influencing the discrimination ability, nor did ants respond differently to neighbors in comparison to non-neighbors.