子宫内膜癌组织中生长抑素受体、VEGF的表达及其与血管形成的关系

目的探讨生长抑素受体（somatostatin receptor，SSTR）、血管内皮生长因子（vascular endothelial growth factor，VEGF）在子宫内膜癌组织中的表达及其与肿瘤血管形成的关系。方法应用免疫组织化学方法检测60例子宫内膜癌组织中SSTR各亚型、VEGF及CD34标记的微血管密度（microvessel denisity，MVD）的表达情况，探讨其与子宫内膜癌临床病理学特征及肿瘤血管形成的关系。结果在60例子宫内膜癌组织中，SSTR各亚型（SSTR1、SSTR2、SSTR3、SSTR4及SSTR5）的阳性表达率分别为70．0％，15．0％。21．7％，23．3％及18．3％；SSTR3、SSTR4在中高分化组表达阳性率明显高于低分化组（P〈0．05）。VEGF的阳性表达率为83．3％，VEGF在低分化组表达阳性率明显高于中高分化组、深肌层浸润组表达阳性率明显高于浅肌层浸润组、FIGO分期≥II期组表达阳性率明显高于I期组（P〈0．05）。子宫内膜癌组MVD（44．85±15．78）明显高于正常子宫内膜组MVD（18．96±4．30）（P〈0．01）。SSTR5的表达与VEGF呈负相关，VEGF阳性表达组子宫内膜癌组织MVD高于VEGF阴性组。结论联合检测SSTR和VEGF对子宫内膜癌预后的评估有一定临床意义。生长抑素类似物（somatostatin analogs，SSTA）可能为子宫内膜癌的诊治提供新的靶点。

Expression of Somatostatin Receptor and VEGF in Endometrial Car- cinoma and Their Relationship to Angiogenesis

Objective To investigate the expression of somatostatin receptor （SSTR） and VEGF in endometrial carcinoma and their relationship to angiogenesis. Methods The expression of SSTR, VEGF in the paraffin-embedded section of 60 cases of endometrial carcinoma and 26 cases of normal endometrium was evaluated by immunohistoehemistry technique. Microvessel density （MVD） of endometrial carcinoma and normal endometrium was also determined with anti-CD34 as the mak- er. The relation between expression of SSTR and VEGF to the clinicopathological parameters and the expression of CD34 were analyzed. Results The positive rate of SSTR subtypes in 60 endometrial cancers was 70 % for SSTR1, 15 % for SSTR2, 21.7 % for SSTR3, 233 % for SSTR4 and 18.3 % for STR5, respectively. The expression of SSTR3, SSTR4 in middle and well differentiated groups was significantly higher than that in poorly differentiated group （ P 〈 0. 05 ） . The positive rate of VEGF in endometrial carcinoma （83.33 % ） was higher than in normal endometrium （30. 76 % ） （ P 〈 0.05 ） . The level of VEGF expression was significantly associated with clinical stage, histological grade and the depth of invasion myometrium （ P 〈 0.05 ） . The positive rate of MVD in endometrial carcinoma was higher than that in normal endometrium. SSTR5 shwoed ~egative correlation with VEGF, while VEGF positive expression was highly correlated with MVD. Conclusion Expressions of SSTR and VEGF are related with prognostic assessment in endometrial carcinoma. A subgroup of receptor-positive endometrial cancers may be a potential target for treatment with somatostatin receptor subtype analogs （SSTA） .