| miR-125b在前列腺癌1E8细胞运动转移中的作用及其分子机制的初步探讨 |
| |
| 引用本文： | 孔繁飞,孙朝阳,王中显,吕煊,翁丹卉,卢运萍,陈刚,李科珍. miR-125b在前列腺癌1E8细胞运动转移中的作用及其分子机制的初步探讨[J]. 国外医学:分子生物学分册, 2011, 0(3): 194-197 |
| |
| 作者姓名： | 孔繁飞 孙朝阳 王中显 吕煊 翁丹卉 卢运萍 陈刚 李科珍 |
| |
| 作者单位： | 华中科技大学同济医学院附属同济医院妇产科,武汉市430030 |
| |
| 基金项目： | 国家重点基础研究发展规划项目（973计划）（No.2009CB521808）,湖北省自然科学基金（No.2010cdb09403） |
| |
| 摘 要： | 目的 研究 miR-125b在前列腺癌高低转移潜能细胞中的表达差异及其对高转移细胞株1E8细胞的运动转移中的作用和可能的分子机制.方法 realtime PCR法检测前列腺癌高低转移潜能配对细胞系中 miR-125b的表达差异.通过划痕实验及transwell实验观察1E8细胞及转染 miR-125b 抑制剂及其阴性对照后该细胞运动转移能力的变化.结果 realtime PCR结果显示高转移潜能1E8细胞中miR-125b表达水明显高于低转移潜能2B4细胞;下调miR-125b会减弱1E8细胞的运动转移能力.结论 miR-125b可促进前列腺癌细胞的运动转移能力.
|
| 关 键 词： | 前列腺癌 miR-125b microRNA |
Effects of MiR-125b on Cell Migration and Invasion in Prostate Cancer Cells |
| |
| KONG Fanfei,SUN Chaoyang,WANG Zhongxian,LV Xuan,WENG Denhui,LU Yunping,CHEN Gang,LI Kezhen. Effects of MiR-125b on Cell Migration and Invasion in Prostate Cancer Cells[J]. , 2011, 0(3): 194-197 |
| |
| Authors: | KONG Fanfei SUN Chaoyang WANG Zhongxian LV Xuan WENG Denhui LU Yunping CHEN Gang LI Kezhen |
| |
| Affiliation: | (Cancer Biology Research Center, Tongfi Hospital, Tongfi Medical College, Huazhong University of Science and Technology, Wuhan , 430030, China) |
| |
| Abstract: | Objective To investigate the differential expression of miR-125b in various prostate cancer cell lines and the effects of miR-125b on cell migration and invasion in prostate cancer. Methods The differential expression of miRNA in 1 E8 （ high metastasis prostate cancer） and 2B4 （low metastasis prostate cancer） cell lines were analyzed by real-time PCR. Cell migration and invasive ability were detected by Transwell experiment. Results The levels of miR-125b significantly increased, compared to 2B4 cells. The invasiveness of 1E8 cells were decreased when miR-125b was down-regulated. Conclusion miR-125b enhances invasiveness of prostate cancer cells. |
| |
| Keywords: | prostate cancer miR-125b microRNA |
| 本文献已被 维普 等数据库收录！ |
