| AlphaLISA技术的发展及应用 |
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| 引用本文： | 王颖,宋娟,崔雨,甘星,孙鹏,王克霞,韩俊,董小平.AlphaLISA技术的发展及应用[J].国外医学：分子生物学分册,2011(3):261-264. |
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| 作者姓名： | 王颖 宋娟 崔雨 甘星 孙鹏 王克霞 韩俊 董小平 |
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| 作者单位： | [1]安徽理工大学医学院,安徽省淮南市232001 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京市102206 |
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| 基金项目： | 国家自然科学基金（No.30771914,30800975）,国家重大传染病防治科技重大专项（No.2009ZX10004-101,2008ZX10004-001,2008ZX10004-002） |
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| 摘 要： | AlphaLISA技术是一种基于微珠的化学发光的新型均相检测技术,与传统的ELISA技术相比具有更高的敏感性、精确性、均一性、背景低、广泛的动态检测范围,而且无需洗涤及样本需求量极少等特点.该技术可用于多种组织、细胞来源的生物分子的检测,如细胞因子、抗原抗体的检测、蛋白相互作用及蛋白质与核酸相互作用的检测以及药物分析等研究.随着AlphaLISA技术的发展,该技术已在医学及分子生物学领域得到了广泛的应用.
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| 关 键 词： | AlphaLISA技术 分子检测 |
Development and Application of AlphaLISA Technology |
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| Institution: | WANG Ying(1 Anhui University of Science and Technology, Medical College, Huainan, Anhui, 232001, China 2State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Dis- ease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China) |
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| Abstract: | The AlphaLISA technology is a bead-based amplified luminescent proximity homogeneous and no-wash assay. AlphaLISA assays are characterized with high sensitivity, low background, and wide dynamic ranges. The new-generation of AlphaLISA technology has been success- fully used in detection of cytokines, antigens, antibodies, protein-protein interaction, protein-nu- cleotide interaction, and drug analysis in medical and molecular biological fields. This review will summarize the principles, advantages, the development and applications of AlphaLISA technology. |
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| Keywords: | AlphaLISA technology molecular detection |
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