High level expression of soluble angiogenin in Escherichia coli |
| |
| Authors: | Yoon J M Kim S H Kwon O B Han S H Kim B K |
| |
| Affiliation: | Department of Microbial Chemistry, College of Pharmacy, Seoul National University, Kwanak-gu. |
| |
| Abstract: | Human angiogenin was genetically engineered and contained the E. coli Omp A signal sequence for secreting soluble angiogenin to the periplasm under tac promoter control. The angiogenin sequence was encoded in a single gene and expressed as a 14.4 kilodalton soluble protein in E. coli. It was purified by CM-Sepharose ion-exchange chromatography and by a heparin-Sepharose affinity chromatography procedure. The biological activity of angiogenin was established by its ability to inhibit mRNA-dependent rabbit reticulocyte cell-free translation. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
