Observation of single-stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy |
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Authors: | Adamcik Jozef Klinov Dmitry V Witz Guillaume Sekatskii Sergey K Dietler Giovanni |
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Institution: | Laboratoire de Physique de la Matière Vivante, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. |
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Abstract: | Atomic force microscopy was used to image single-stranded DNA (ssDNA) adsorbed on mica modified by Mg(2+), by 3-aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base-pairing, thus this method could be important for studying certain processes involving ssDNA in more details. |
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Keywords: | AFM atomic force microscopy ssDNA single stranded DNA HOPG highly oriented pyrolytic graphite |
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