Detection of genetically unstable loci in parthenogenic families of lizards of theLacerta genus by DNA fingerprinting |
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Authors: | N G Kan I A Martyrosyan I S Darevsky F D Danielyan M S Arakelyan A V Aslanyan V V Grechko O N Tokarskaya A P Ryskov |
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Institution: | (1) Institute of Gene Biology, Russian Academy of Sciences, 117334 Moscow, Russia;(2) Institute of Zoology, Russian Academy of Sciences, 199034 St. Petersburg, Russia;(3) Erevan State University, 375000 Erevan, Armenia;(4) Engelhardt Institute of Molecular Biology, 117984 Moscow, Russia |
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Abstract: | Two parthenogenic families of unisexual species of Caucasian rock lizards of genusLacerta, L. armeniaca andL. unisexualis, were analyzed by DNA fingerprinting. Inheritance of M13 minisatellite and of (GACA)
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, (GATA)
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, and (TCC)
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microsatellite loci in the first generation of the lizards was studied. M13, (GACA)
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, and (TCC)
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loci in the families ofL. armeniaca were strictly inherited, as well as M13 and (GACA)
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loci in the families ofL. unisexualis: each DNA fragment in the fingerprint patterns of progeny could be detected in the maternal pattern. However, when a (TCC)50 microsatellite probe was applied in the study ofL. unisexualis families, specific DNA fragments with altered mobility were revealed in the progeny patterns, and the frequency of such events
was rather high. It might be hypothesized that some of the (TCC)
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loci inL. unisexualis genome are highly mutable. Hence, the family analysis allowed us to demonstrate experimentally the presence of genetically
unstable loci in genomes of parthenogenic species of vertebrates. The nature and mechanism of the instability of these loci
in parthenogenesis remain obscure. |
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Keywords: | parthenogenic lizards Lacerta armeniaca Lacerta unisexualis microsatellite minisatellite genetic variability unstable loci |
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