Preparative purification of Escherichia coli heat-stable enterotoxin |
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| Authors: | A M Saeed R N Greenberg |
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| Affiliation: | 1. Hebei Key Laboratory of close-to-Nature restoration technology of wetlands, School of Eco-Environment, Hebei university, Baoding 071002, China;2. School of life science, Hebei university, Baoding 071002, China;3. Institute of Xiong''an New Area, Hebei university, Baoding 071002, China;4. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;5. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China |
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| Abstract: | Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures. |
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