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CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages |
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| Authors: | Richa K Dave David A Hume Caryn Elsegood Stuart Kellie |
| Institution: | a Institute for Molecular Bioscience, The University of Queensland, St Lucia, Qld 4072, Australia b Cooperative Research Centre for Chronic Inflammatory Diseases (CRC-CID), The University of Queensland, St Lucia, Qld 4072, Australia c ARC Special Research Centre for Functional and Applied Genomics, The University of Queensland, St Lucia, Qld 4072, Australia d School of Molecular and Microbial Sciences, The University of Queensland, Qld 4072, Australia e Department of Medicine, University of Melbourne, Melbourne, Victoria, Australia f The Roslin Institute, University of Edinburgh, Roslin EH25 9PS, Scotland, UK |
| Abstract: | In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity. |
| Keywords: | Tyrosine phosphatase Macrophage Cytoskeleton |
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