Modulation of DNA glycosylase activities in mesenchymal stem cells |
| |
Authors: | Gunn A. Hildrestrand,Shivali Duggal,Magnar Bjø rå s,Jan E. Brinchmann |
| |
Affiliation: | a Centre for Molecular Biology and Neuroscience and Institute of Medical Microbiology, Rikshospitalet University Hospital, 0027 Oslo, Norway b Institute of Immunology, Rikshospitalet University Hospital, 0027 Oslo, Norway |
| |
Abstract: | Adipose-tissue derived mesenchymal stem cells (AT-MSCs) are a promising tool for use in cell-based therapies. However, in vitro expansion is required to obtain clinically relevant cell numbers, and this might increase the chance of genomic instability. DNA repair is crucial for maintaining DNA integrity. Here we have compared the initial step of base excision repair in uncultured and cultured AT-MSCs by analysis of base removal activities and expression levels of relevant DNA glycosylases. Uracil, 5-hydroxyuracil and ethenoadenine removal activities were upregulated in cultured cells compared to uncultured cells. In contrast, both the 8-oxo-7,8-dihydroguanine (8-oxoG) removal activity and the concentration of 8-oxoG bases in the DNA were reduced in the cultured cells. Gene expression analysis showed no substantial changes in mRNA expression. The glycosylase activities remained stable through at least 12 passages, suggesting that DNA repair is proficient through the period required for in vitro expansion of AT-MSCs to clinically relevant numbers. |
| |
Keywords: | Mesenchymal stem cells Base excision repair DNA glycosylases 8-oxoG |
本文献已被 ScienceDirect 等数据库收录! |
|