芦苇耐盐变异体与野生型植株某些特性的比较 |
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引用本文: | 许祥明,叶和春.芦苇耐盐变异体与野生型植株某些特性的比较[J].Acta Botanica Sinica,2000,42(11):1126-1130. |
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作者姓名: | 许祥明 叶和春 |
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作者单位: | 中国科学院植物研究所,北京 |
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基金项目: | heNationalOceanHighScitechnologyProgram (863 )ofChina! (819_0 8_0 5 ) |
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摘 要: | 地通过细胞工程方法得到的耐盐芦苇(Phragmites communis Trin.)变异系R5002-12与野生型植株进行了分子生物学和生理生化特性比较分析。筛选到5个RAPD引物对变异体和野生型植株的DNAF的随机扩增表现出不同的多态性,表明该变异体在分子水平上发生了变化。生理生化分析的结果表明,平行生长状态下的两种株系,其可溶性蛋白和同工酶的表达水平和种类不一样,变异体在NaCl胁迫下,表达
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关 键 词: | 芦苇 耐盐变异体 RAPD 同工酶 叶绿素 |
Comparison of Some Characteristics Between Phragmites communis and Its Salt Tolerant Variant |
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Authors: | XU Xiang_Ming YE He_Chun LI Guo_Feng CHEN Jian_Lin [WTBZ] |
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Institution: | XU Xiang_Ming,YE He_Chun *,LI Guo_Feng,CHEN Jian_Lin [WT6BZ] |
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Abstract: | R5002_12, a salt tolerant line of Phragmites communis Trin., which was obtained from ethyl methane sulfonate (EMS) treated callus selected under saline stress, was compared with its wild line in respect to their molecular biological, physiological and biochemical characterizations. Five arbitrary primers were screened which showed differences in DNA amplified polymorphism between the variant and its wild line. Some new proteins appeared in the salt tolerant plant under salt stress. Electrophoresis of peroxidase and esterase also showed some differences in isozyme expression between them. The chlorophyll content of the variant was higher than that of the original variety, whether the plants were under salt stress or not. |
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Keywords: | Phragmites communis salt tolerant variant RAPD isozyme chlorophyll soluble protein |
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