Extremely Rapid Extraction of DNA from Bacteria and Yeasts |
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Authors: | Hai-Rong Cheng Ning Jiang |
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Institution: | (1) Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China;(2) Graduate School, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is
presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform
to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction
enzyme digestion and genomic library construction. This method is reproducible and simple for the routine DNA extraction from
bacteria and yeasts. |
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Keywords: | bacteria DNA extraction yeast |
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