水稻花药对高温胁迫的生理响应

为了探明水稻花药在高温胁迫下的生理反应及适应机理，以耐热水稻品系996和热敏感水稻品系4628为材料，在人工气候室进行高温（9：00-17：00，37℃；17：00-9：00，30℃）和适温处理（9：00-17：00，30℃；17：00～9：00，25℃），研究高温胁迫对水稻花药可溶性糖、可溶性蛋白质、热稳定蛋白、维生素C含量、膜透性及内源激素含量等生理特性的影响。结果表明，高温使水稻花药中可溶性糖含量、可溶性蛋白质含量和维生素C含量下降，热稳定蛋白含量上升；热敏感品系4628花药中丙二醛含量和相对电导率在高温胁迫下增幅大于耐热品系996；高温胁迫下，水稻花药中GA3和IAA含量降低，ABA含量上升，耐热品系996花药中GA3、IAA含量降低幅度小于热敏感品系4628，ABA含量增加幅度小于热敏感品系4628。

Physiological Responses of Anther to High Temperature Stress in Rices

In order to ascertain the physiological reaction and mechanism of adaption to high temperature stress of anther in rice, we treated heat-tolerant line 996 and heat-sensitive line 4628 with high temperature （9：00- 17：00, 37 ℃; 17：00-9：00, 30 ℃） treatment and optimal temperature （9：00-17：00, 30 ℃; 17：00-9：00, 25 ℃） treatment in the growth chambers. The soluble sugar content, soluble protein content, heat stable protein content, vitamin C content, membrane permeability and endogenous hormone content of anther in rice were studied. The results showed that soluble sugar content, soluble protein content and vitamin C content of anther decreased under high temperature stress, but heat stable protein content increased; The increase scope of malondialdehyde （MDA） content and relative conductivity in heat-sensitive line 4628 was greater under high temperature stress than that of in heat-tolerant line 996; Under high temperature stress, GA3 and IAA content of anther decreased, while ABA content of anther increased, the decreasing scope of GA3 and IAA content in heat tolerant line 996 was less than that of heat-sensitive line 4628, the increasing scope of ABA content was less than that of heat-sensitive line 4628.