Plantlet production through high frequency somatic embryogenesis in long term cultures ofEucalyptus citriodora |
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Authors: | E M Muralidharan P K Gupta A F Mascarenhas |
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Institution: | (1) Division of Biochemical Sciences, National Chemical Laboratory, 411 008 Pune, India |
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Abstract: | A highly embryogenic culture ofEucalyptus citriodora was obtained by repetitive embryogenesis from somatic embryos cultured in the dark on a medium containing 500 mg/l each of glutamine and casein hydrolysate, 30 g/l of sucrose and 5 mg/l of 1-napthaleneacetic acid. Cultures retained morphogenetic ability for upto 36 months when maintained at 27°C by subculture at intervals of 4–5 weeks. The subculture period could be extended beyond 9 months if cultures were incubated at 10°C. On a hormone free medium incubated in light 50% of the embryos germinated to plantlets of which 70% survived when transferred to a sand and soil mixture.Abbreviations NAA
1-naphthal eneacetic acid
NCL Communication No: 4480 |
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