Quantification of a potent mutagenic 4-amino-3,3′-dichloro-5,4′-dinitrobiphenyl (ADDB) and the related chemicals in water from the Waka River in Wakayama, Japan |
| |
Authors: | Tomoko Mizuno Takeji Takamura-Enya Tetsushi Watanabe Tomohiro Hasei Keiji Wakabayashi Takeshi Ohe |
| |
Affiliation: | aDepartment of Food and Nutrition, Faculty of Home Economics, Kyoto Women's University, Kyoto 605-8501, Japan;bDepartment of Applied Chemistry, Faculty of Engineering, Kanagawa Institute of Technology, Kanagawa 243-0292, Japan;cDepartment of Public Health, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan;dCancer Prevention Basic Research Project, National Cancer Center Research Institute, Tokyo 104-0045, Japan |
| |
Abstract: | 4-Amino-3,3′-dichloro-5,4′-dinitrobiphenyl (ADDB) is a novel chemical exerting strong mutagenicity, especially in the absence of metabolic activation. In addition to mutagenicity, ADDB may also disrupt the endocrine system in vitro. ADDB may be discharged from chemical plants near the Waka River and could be unintentionally formed via post-emission modification of drainage water containing 3,3′-dichlorobenzidine (DCB), which is a precursor in the manufacture of polymers and dye intermediates in chemical plants. The main purpose of this study was to make a comprehensive survey of the behaviour and levels of ADDB and suspected starting material or intermediates of ADDB, i.e., DCB, 3,3′-dichloro-4,4′-dinitrobiphenyl (DDB), and 4-amino-3,3′-dichloro-4′-nitrobipheny (ADNB) in Waka River water samples. We also postulated the formation pathway of ADDB. Water samples were collected at five sampling sites from the Waka River four times between March 2003 and December 2004. Samples were passed through Supelpak2 columns, and adsorbed materials were then extracted with methanol. Extracts were used for quantification of ADDB and the related chemicals by HPLC on reverse-phase columns; mutagenicity was evaluated in the Salmonella assay using the O-acetyltransferase-overexpressing strain YG1024. High levels of ADDB, DCB, DDB, and ADNB (12.0, 20,400, 134.8, and 149.4 ng/L-equivalent) were detected in the samples collected at the site where wastewater was discharged from chemical plants into the river. These water samples also showed stronger mutagenicity in YG1024 both with and without S9 mix than the other water samples collected from upstream and downstream sites. The results suggest that ADDB is unintentionally formed from DCB via ADNB in the process of wastewater treatment of drainage water containing DCB from chemical plants. |
| |
Keywords: | 4-Amino-3 3′ -dichloro-5 4′ -dinitrobiphenyl (ADDB) 3 3′ -Dichlorobenzidine (DCB) 3 3′ -Dichloro-4 4′ -dinitrobiphenyl 4-Amino-3 3′ -dichloro-4′ -nitrobiphenyl Salmonella mutagenicity assay River water |
本文献已被 ScienceDirect 等数据库收录! |
|