Characterization of Cfa1, a monofunctional acyl carrier protein involved in the biosynthesis of the phytotoxin coronatine |
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| Authors: | Seidle Heather Rangaswamy Vidhya Couch Robin Bender Carol L Parry Ronald J |
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| Institution: | Department of Chemistry, Rice University, Houston, Texas 77005, USA. |
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| Abstract: | Cfa1 was overproduced in Escherichia coli and Pseudomonas syringae, and the degree of 4'-phosphopantetheinylation was determined. The malonyl-coenzyme A:acyl carrier protein transacylase (FabD) of P. syringae was overproduced and shown to catalyze malonylation of Cfa1, suggesting that FabD plays a role in coronatine biosynthesis. Highly purified Cfa1 did not exhibit self-malonylation activity. |
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