Clustering of the trp genes in Burkholderia (formerly Pseudomonas) cepacia |
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| Authors: | Hideki Matsumoto Kakuya Furihata Makoto Ohnishi Bruce W Holloway |
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| Institution: | Department of Bacteriology, Shinshu university. School of Medicine, Matsumoto 390, Japan; Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria 3168, Australia |
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| Abstract: | Abstract Chromosomal location of trp genes of a strain Burkholderia cepacia (formerly Pseudomonas cepacia ) has been determined by transduction using a generalized transducing phage CP75 and by molecular analysis for a cosmid plasmid clone with trp genes isolated from the genomic gene library of the strain. The trp genes were classified into three linkage groups and they all were closely linked on a short chromosomal region probably in the order ( trpA, trpB, trpF)-(trpC, trpD)-trpE . |
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| Keywords: | Burkholderia cepacia trp genes Chromosomal location Transduction Phage CP75 |
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