C－反应蛋白与模型受体的特异性结合

Ｃ－反应蛋白是动物体内一种典型的急性期反应蛋白。本文人工合成了两种可以与Ｃ－反应蛋白特异性结合的兼性分子作为Ｃ－反应蛋白的模型受体，以便进一步在脂单层膜表面上组装Ｃ－反应蛋白的二维晶体。作为第一步工作，本文研究了兼性分子的特性以及荧光光谱方法监测兼性分子与Ｃ－反应蛋白之间特异性相互作用。荧光光谱实验结果表明受体与Ｃ－反应的特异结合会引起荧光强度的下降。

THE SPECIFIC BINDING OF C-REACTIVE PROTEIN WITH MODEL RECEPTOR

C-reactive protein is one of the most characteristic proteins in the acute phase, which partcipates in the acute inflammatory reactions. The synthetic amphiphiphilic molecules were used as the membrane bound model rceptors of the C-anctive protein in the present work. The specific binding between C-reactive protein and the amphiphilic molecules was studied, which is the first step towards the two-dimensional crystallization of C-reactive protein on the lipid monolayer. The fluorescence spectra showed that the fluorescence intensity of C-reactive protein was decreased after spacifically binding with its receptors.