Cloning of the Glutamyl-tRNA Synthetase (gltX) Gene from Pseudomonas aeruginosa |
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| Authors: | Clifton V Franklund and Joanna B Goldberg |
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| Institution: | Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA. |
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| Abstract: | The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti. |
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