Purification and properties of cyclic AMP dependent and independent protein kinases from rat pancreas. |
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Authors: | Y Cenatiempo P Mangeat G Marchis-Mouren |
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Affiliation: | Institut de Chimie Biologique, Université d''Aix-Marseille, Place Victor-Hugo, 13331 Marseille Cédex 3 (France) |
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Abstract: | Three protein kinases Ko, K1, and KII have been extracted from rat pancreas homogenate, Ko is not stimulated by cyclic AMP. K1 is poorly stimulated by cyclic AMP (1.3 times), Ku is highly stimulated (6 times). The specificity of KII with respect to various nucleotides and cyclic nucleotides has been determined. K1 and KII account for the total cyclic AMP dependent protein kinase activity in the homogenate. |
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Keywords: | To whom all correspondence should be addressed. |
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