| 限制和修饰系统LlaBIII在构建抗噬菌体菌株中的作用 |
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| 引用本文： | 孔健 Jytte Josephsen 马桂荣.限制和修饰系统LlaBIII在构建抗噬菌体菌株中的作用[J].微生物学报,2002,42(2). |
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| 作者姓名： | 孔健 Jytte Josephsen 马桂荣 |
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| 作者单位： | 山东大学微生物技术国家重点实验室 济南 250100；丹麦皇家畜牧农业大学乳制品和食品科学系 丹麦 |
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| 摘 要： | 限制和修饰 (ｒｅｓｔｒｉｃｔｉｏｎａｎｄｍｏｄｉｆｉｃａｔｉｏｎ ,Ｒ Ｍ)系统是指由限制性内切酶和甲基化酶组成的单亚基或多亚基复合酶系统 ,两者通常成对出现 ,具有相同的ＤＮＡ识别位点 ,其作用相反。Ｒ Ｍ系统在原核生物中普遍存在 ,在保护细胞免遭外源病毒侵害方面具有重要作用1] 。作为发酵剂的乳酸乳球菌在乳制品发酵中具有重要作用 ,但这类菌株极易遭受噬菌体感染 ,导致菌株产酸力降低 ,甚至发酵失败 ,造成严重的经济损失。所以在乳制品发酵过程中防止噬菌体感染就成为十分重要的问题。通过自然筛选或诱变处理等手段筛选噬菌…
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| 关 键 词： | 乳酸乳球菌， 噬菌体， 限制和修饰系统， 乳制品发酵 |
The Resistance Conferred by the R/M System LlaBIII Against Bacteriophages |
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| Kong Jian,Jytte Josephsen,Ma Gui-Rong.The Resistance Conferred by the R/M System LlaBIII Against Bacteriophages[J].Acta Microbiologica Sinica,2002,42(2). |
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| Authors: | Kong Jian Jytte Josephsen Ma Gui-Rong |
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| Institution: | Kong Jian 1 Jytte Josephsen 2 Ma Gui-Rong 1 |
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| Abstract: | LlaBIII,isolated from the naturally occurring plasmid pJW566 from Lactococcus lactis subsp.cremoris W56,encoded a restriction and modification (R/M) system. The plasmid pJK1 carrying the R/M system LlaBIII was transformed into L.lactis IL1403 with type I R/M system located on chromosome and the strain MG1614pAW601] with AbiS gene on plasmid pAW601, respectively. The transformants obtained showed stacking resistance against bacteriophages. The plasmid pJK1 was transformed into industrial strains L.lactis SMQ86 and CHCC2281,the transformants showed the EOP of the bacteriophages decreased by 10 -3 and 10 -5, respectively.The results indicated that the R/M system LlaBIII could protect strains from bacteriophages in dairy fermentation. |
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| Keywords: | Lactococcus lactis Bacteriophages Restriction and modification system Dairy fermentation |
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