Preparative purification of dipeptidyl peptidase IV. |
| |
Authors: | R Seidl W Schaefer |
| |
Affiliation: | Max-Planck-Institute for Biochemistry, Martinsried. |
| |
Abstract: | Dipeptidyl-Peptidase IV was purified from pig kidney by ammonium sulfate fractionation, gel filtration, QAE-cellulose chromatography and affinity columns with Gly-Pro- and Concanavalin A-Sepharose. The specific activity of the purified enzyme is 41.8 units/mg. Polyacrylamide gel electrophoresis and silver staining show a single band. The enzyme preparation is free of aminopeptidase and dipeptidase activity, proved fluorimetrically and by gas chromatography/mass spectrometry. The most important procedure for removal of contaminating enzyme activities is a stepwise NaCl-gradient on a QAE-ZetaPrep ion exchange disk. |
| |
Keywords: | |
|
|