Glucose transport and metabolism in Gymnodinium breve |
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Authors: | Daniel G Baden Thomas J Mende |
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Institution: | University of Miami, Department of Biochemistry, P.O. Box 520875, Miami, FL 33152, U.S.A. |
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Abstract: | Florida's red tide organism, Gymnodinium breve, utilized exogenous glucose in the light for the synthesis of cellular components. Glucose was not taken up in the dark. Kinetic parameters for glucose uptake include a KFD of 11 μM and a Vmax of 1 × 10?10 mol of glucose taken up/mg cellular protein/hr. Glucose uptake was competitively inhibited by phloridzin (Ki = 40 μM), mannose (Ki = 12O μM), and 2-deoxy-d-glucose (Ki = 190 μM) and non-competitively inhibited by galactose (Ki = 125 μM). Kinetics and inhibition of glucose uptake are consistent with a facilitated diffusion transport system. |
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Keywords: | dinoflagellate Florida red tide glucose transport and metabolism |
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