Stereospecificity of cinnamyl alcohol dehydrogenase and synthesis of stereospecifically labelled coniferyl alcohol |
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Authors: | Martina Klischies Joachim Stöckigt Meinhart H. Zenk |
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Affiliation: | Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D 4630 Bochum, West Germany |
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Abstract: | Using horse liver alcohol dehydrogenase, stereospecifically tritiated (R)- and (S)-(γ-3H)-coniferyl alcohol was synthesized. Using both of these substrates it was demonstrated that cinnamyl alcohol dehydrogenase from lignifying Forsythia tissue specifically removes the pro-R-hydrogen atom of coniferyl alcohol in the oxidation to the aldehyde. This also means that in the reverse reaction the A-hydrogen of NADPH is transferred to the Re-site of coniferyl aldehyde. |
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Keywords: | Oleaceae cinnamyl alcohol oxido-reductase cinnamyl alcohol cinnamyl aldehydes stereospecificity. |
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