Monitoring human exposure to carcinogens by DNA adduct measurement |
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| Authors: | Regina M Santella |
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| Institution: | (1) Cancer Center and Division of Environmental Sciences School of Public Health, Columbia University, New York, New York;(2) Cancer Center and Division of Environmental Sciences, School of Public Health, Columbia University, New York, NY |
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| Abstract: | Sensitive immunologic techniques are now available for the detection and quantitation of carcinogen-DNA adducts. We have developed a number of specific monoclonal antibodies which recognize DNA modified by particular carcinogens, including benzoa]-py-rene, aminopyrene, 8-methoxypsoralen plus UVA light and vinyl chloride. These antibodies can be used in competitive enzyme-linked immunosorbent assays to quantitate adducts in DNA isolated from biological samples. Samples from treated animals as well as from humans with occupational or environmental exposure to carcinogens have been studied. In addition, antibodies can be used in indirect immunohistochemical studies to localize adduct formation in various tissues or cell types.Abbreviations BPDE-1
7 ,8 -dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzoa]pyrene
- CTCL
Cutaneous T cell lymphoma
- ELISA
enzyme linked immunosorbent assay
- 8-MOP
8-methoxypsoralen
- PUVA
8-MOP plus UVA irradiation |
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| Keywords: | adducts aminopyrene benzo[a]pyrene exposure 8-methoxypsoralen monoclonal antibodies UVA irradiation vinyl chloride |
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